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KMID : 0617320010100010085
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Journal of Pharmacetical Sceiences Ewha Womans University
2001 Volume.10 No. 1 p.85 ~ p.90
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Identification of Structural Domains Affecting Transactivation Potential of Nm23
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Cho, Seong-Jun
Lee, Nam-Sihk/Jung, Yong-Sam/Lee, Hansoo/Lee, Kong-Joo/Kim, Eunhee/Chae, Suhn-Kee
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Abstract
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The strong transactivation activity of the C-terminal half(amino acids 76-152) of Nm23 was reported previously. Here we examined a structural domain preventing or necessary to its transactivation activity. The C-terminal 1/4 (amino acids 109-052) was sufficient form transactivation, but the C-terminal half with a longer N-terinal extension (amino acids 58-152) caused the loss of the transactivation ability. Furthermore, co-expression of the N-termanal half with the C-terminus of Nm23-H1 blocked the transactivation activity of the C-terminal half, where direct interaction of both truncated proteins was demonstrated in vitro. Transactivation activities in the C-terminal halves of the known mutants (P96S, H118F, S120G, and S120A) exhibiting differential antimetastasis effects were also tested. Significant reduction of transactivation activity was observed only in H118F, indicating that NPD kinase active-site histidine is required. This suggests that transactivation potential of Nm23 is related to NDP kinase activity but not to metastasis suppressor activity.
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KEYWORD
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